Does the MultiPep with SPOT upgrade kit require a computer?
The system is operated from a standard PC. The graphical software takes care of derivative and sequence definition, generation of peptide sequences from parent protein sequences, calculation of reagent consumption, operation of the instrument and generation of a log file of operation.
How much programming is required to operate the upgrade MultiPep?
During everyday use you just define the peptide sequences, select a protocol, load the work area and start the synthesis. More experienced users can modify the pre-defined protocols and adapt them to their specific needs. The relevant parameters of the protocol are accessible in the software.
Do I have access to all synthesis parameters?
The synthesis cycle is composed of individual tasks of the robot. The software lets you have full access to all relevant parameters and you can modify existing methods or create new procedures.
Is there a record of what the instrument does?
All instrument operations are automatically recorded to the computer hard disc. The files can be opened by standard text editors.
What are the features of the software?
The software allows definition and handling of peptide sequences in a simple text format. Protein sequences entered in single letter code can be translated into overlapping peptides, and peptide libraries are easily generated from parent sequences by a few simple definitions.
As automation of the SPOT synthesis procedure allows to handle large arrays, a powerful software is necessary to generate the sequences. Sequences can be entered in the software itself or imported from text files or from other sources. There are several modes to generate peptides from parent protein or peptide sequences:
- individual peptides (entered one by one)
- analogue sequences (by defined amino acid replacement)
- overlapping peptides (generated from a protein sequence)
- overlapping peptides of different length (from a protein sequence)
- peptide libraries with a maximum of two mixed positions (libraries)
