The SPOT method was originally developed by Ronald Frank and can be used to generate libraries of peptides for a wide range of applications. Peptides are synthesized by repeated deposition of activated amino acids as spots on a specially derivatized filter sheet. The filter must be deprotected and washed extensively in each synthesis cycle.

To fully automate SPOT synthesis an upgrade kit with hardware and software is available for the MultiPep synthesizer. Two membranes are mounted in a steel frame on top of a supporting Teflon foil with extraction holes. The solid construction allows to fix the synthesis membrane on all sides with no leakage of reagents or solvents. Solvents are distributed by a manifold and extracted by vacuum. Vacuum extraction is performed rapidly and the membranes can be fully dried after washing with ethanol. An optimized chemistry has been established to provide quality peptide sets at low cost on a routine basis. Up to 800 peptides can be synthesized on two microtiter plate sized synthesis membranes. They remain bound to the membrane and can be compared in a single assay for their biological activity. 

Please note: The SPOT synthesis method is subject to special licensing conditions and not available for commercial applications.