- synthesis of 20 mg peptide of up to 15 amino acids length, purification up to 95% (HPLC)  - conjugation of 2 mg peptide to 2 mg KLH, and a smaller amount to OVA for testing  - one ELISA plate coated with individual peptide-ovalbumin-conjugate  - immunization of two animals with the peptide-KLH-conjugate  - ELISA test with data sheet of serum samples obtained after the second boost injections

- synthesis of 2 - 4 custom peptides of up to 15 amino acids length, purification up to 95% (HPLC)  - individual coupling of each peptide to KLH, and a smaller amount to OVA for testing  - one ELISA plate coated with individual peptide-ovalbumin-conjugates  - immunization of two animals with the pooled peptide-KLH-conjugates  - ELISA test with data sheet of serum samples obtained after the second boost injections

ELISA tests are performed with serum samples obtained after the second boost injections. The ELISA plate is coated with peptide-ovalbumin-conjugates.

Purification of serum is available upon request. For the antibody purification a column is used to which the peptide that was taken for immunization is covalently bound.