What is the minimum amount of protein identified with the DigestPro MS?
As this depends on too many variables we specify the sensitivity as follows:
Standard proteins like BSA can be detected after digestion if 0.5 pmol were loaded onto a gel and processed. As a rule of thumb:

•  Proteins visualized with Coomassie Blue can be identified if they are in a database.
• Spots or bands from silver gels work in may but not all cases.

Does INTAVIS you supply reagents?
As high quality buffers are available from several sources, INTAVIS does not supply reagents. However, we supply reaction and collect plates as well as other consumables.

Can the DigestPro MS be used for other chemical derivatization?
As long as the principle for reagent incubation and exchange is the same the DigestPro MS can be programmed for other derivatization reactions.

Can other enzymes than trypsin be used?
The DigestPro MS has three reservoir positions for enzymes. If the protocols are compatible these can even be used in the same run.

Can the DigestPro MS be used to digest proteins in solution?
As soluble proteins not contained in a gel matrix can not be retained in the flow-through vessels they can not be processed like gel pieces. However, there are protocols for digestion of soluble proteins loaded onto Millipore ZipTips, which can be run on the MALDI part of the DigestPro MS.