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Manuals
As the InsituPro Upgrade is basically an InsituPro with the mode of action and operation software of the InsituPro VS, we donīt have a specific manual. For technical details please see the InsituPro Manual, for application and software please see the InsituPro VS manual.
InsituPro VS Manual 2.0
Recycling of baskets Protocol of the Month December 2007 Method development Template Protocols
Basically, you will find 2 protocols for IHC and ISH each for each configuration (small and medium baskets) and one clean protocol. The protocols are the same as for the InsituPro VS. Protocol clean Method: Sterilizing the tubings and baskets This protocol should be used to clean up the incubation baskets prior to their first use. The baskets will be sterilized by rinsing them with 3% hydrogenperoxide at elevated temperature. Small baskets PDF Medium baskets PDF Template protocol ISH 1 Method: In situ hybridisation This protocol can be used as a template for seamless adaptation of short manual in situ hybridization protocols. The template includes prehybridization and hybridization steps as well as the post hybridization washes, all performed at elevated temperature. The incubation with DIG antibody and intensive washing steps are included as well. Small baskets PDF Medium baskets PDF Template protocol ISH 2 Method: In situ hybridisation This protocol can be used as a template for seamless adaptation of manual in situ hybridization protocols. It already includes most steps of a typical in situ hybridization method like rehydration of the specimen, bleaching with hydrogenperoxide, permeation using proteinase K and postfixation. The prehybridization and hybridization steps as well as the post hybridization washes are performed at elevated temperature. Small baskets PDF Medium baskets PDF Template protocol ICC 1 Method: Immunodetection This protocol can be used as a template for seamless adaptation of short manual in situ immunostains. The template includes incubation with primary antibody and secondary antibody as well as all washing steps between and after these two incubations. Most steps are performed below ambient temperature. Small baskets PDF Medium baskets PDF Template protocol ICC 2 Method: Immunodetection This protocol can be used as a template for seamless adaptation of manual in situ immunostains. The template already includes most steps of a typical immunocytochemical method like rehydration of the specimen, permeation using proteinase K and postfixation. The incubation with primary antibody and secondary antibody as well as all washing steps between these two incubations, are performed at a temperature below ambient. Small baskets PDF Medium baskets PDF |
